Abstract: Low carbon tourism, as an emerging sustainable tourism method, is a new tourism model that aims to reduce carbon emissions and protect the ecological environment. The study takes wildlife tourism as the starting point, focuses on the low-carbon behavior of tourists during the tourism process, explores the influencing factors of low-carbon behavior of tourists in this scenario, and emphasizes the key role of tourists in achieving low-carbon tourism and promoting sustainable development of tourist destinations. Selecting tourists from Chengdu Giant Panda Breeding Base as the research object, a structural equation model is constructed to explore the relationship between tourists′ low-carbon tourism motivation, low-carbon tourism attitude, low-carbon tourism environment, low-carbon environmental education, low-carbon tourism cognition, low-carbon tourism participation willingness, and low-carbon tourism behavior. The research results show that the low-carbon tourism motivation, low-carbon tourism attitude, and low-carbon environmental education received by tourists all have a significant impact on low-carbon tourism behavior. When tourists harbor emotional factors such as a desire to be close to nature, sympathy for nature, and a sense of environmental responsibility, it can effectively stimulate their low-carbon behavior. The low-carbon tourism environment and low-carbon tourism cognition did not have a significant positive impact on low-carbon tourism behavior. When making tourism decisions, tourists pay more attention to the tourism experience rather than low-carbon environmental factors, and some tourists may believe that low-carbon behavior conflicts with the tourism experience. Therefore, choose to avoid or reduce low-carbon behavior. This study aims to enrich the research perspective of low-carbon tourism and provide case support for the development of low-carbon tourism in national parks and nature reserves.