Abstract: As an essential measure for managing the marine ecosystem, scientifically effective spatial planning of marine protected areas can enhance the protection of marine biodiversity and ecosystem health. The application prospects of the Object-oriented simulator of marine ecosystem exploitation (OSMOSE) in marine protected areas spatial planning are discussed. An in-depth analysis of the model principle, key processes, and research progress, along with its features and functional realization, highlights the various advantages the OSMOSE model can offer in marine protected areas spatial planning and through practical case analysis, the similarities, differences, and potential integrative possibilities between the OSMOSE model and established spatial planning techniques are proposed. The results indicate that the OSMOSE model demonstrates significant advantages in simulating marine ecosystems dynamics and predicting the effectiveness of management strategies. The proposed model can precisely simulate multi-species interactions and spatiao-temporal distributions, thus providing rich data support and a decision basis for marine protected areas spatial planning. The rational application of the model not only provides a new perspective for constructing marine protected areas in China but also enhances the rationality of spatial planning and the effectiveness of management measures in marine protected areas, playing a crucial role in promoting, China′s marine ecological conservation.