Abstract: The goal of ecological civilization construction is to achieve harmonious coexistence of humans and nature. Alleviation of human-wildlife conflict and realization of man-nature harmonious coexistence is an important issue to be solved urgently. From the perspective of event geography, this paper collected 467 human-wildlife conflict events reported in Tiktok short videos through big data, extracted the time and location of human-wildlife conflict events, and analyzed the spatio-temporal characteristics of human-wildlife conflict occurrence via monthly index, geographic concentration index and ArcGIS visualization. Social semantic network analysis and grounded theory qualitative analysis were conducted on the short video comments, From the integrated interactive perspective of "human-habitat-wildlife", a model framework of human-wildlife conflict formation mechanism was constructed. As shown by the results, the human-wildlife conflicts occurred most frequently in May and November nationwide, and geographically showed a large scale, multi-point distribution with a relatively low degree of concentration. According to the conflict mechanism analysis, 15 original categorizations were obtained. Through further coding, it could be found that the occurrence of human-wildlife conflict events was resulted from the joint action of "human-habitat-wildlife". On this basis, experiences and methods for mitigating human-wildlife conflicts were proposed to promote the man-nature harmonious coexistence.